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Test Code CDIF C DIFFICILE TOXIN, PCR

Important Note

Not performed on children less than 2-years old.

Due to sensitivity of the assay, only one sample will be run in 7 days.

One specimen is sufficient for diagnosis of bacterial gastroenteritis and antibiotic associated diarrhea/pseudomembranous enterocolitis (Clostridium difficile infection).

Consult laboratory if infection is strongly suspected despite a negative culture and negative C. difficile toxin.

Performing Laboratory

Porter Hospital Laboratory

Specimen Requirements

Specimen Source: Feces

Soft or liquid stool - sample must take the

shape of the container

Collection Container/Tube: Stool container

Submission Container/Tube: Plastic container

Collection Instructions:

1. Must be transported in a clean container, no

    preservatives, and refrigerated within 2

    hours of collection.
2. Formed stools are not acceptable. 

3. The sample should be tested as soon as possible,

    but may be held up to two days at 2-8 C prior to testing.

4.  If testing cannot be performed within this time frame,

     samples should be frozen immediately on receipt and stored frozen (-20° C) until tested.

Specimen Transport Temperature

Refrigerate

Days Performed

Daily / Not available STAT

Methodology

PCR

Test Classification and CPT Coding

87493

Normal Reference Values

Negative for C. difficile by PCR.
Presumptive negative for 027, NAP1 B1 strain.

Critical Values

Positive (Results are called.)