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Test Code CYTH1 T-Helper Cell Type 1 Cytokine Panel, Plasma


Specimen Required


Supplies: Sarstedt Aliquot Tube, 5 mL (T914)

Collection Container/Tube: Lavender top (EDTA)

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions:

1. Immediately after specimen collection, place the tube on wet ice.

2. Within 2 hours of collection, centrifuge at 4° C, 1500 x g for 10 minutes and aliquot plasma into a plastic vial

3. Freeze immediately.

Additional Information:

1. Plasma for this test cannot be shared with other tests. Submit specimen for this test in its own vial.

2. This test cannot be added on to a previously collected specimen.


Useful For

Assessment of plasma cytokine levels to understand the etiology of autoimmune and proinflammatory conditions when used in conjunction with clinical information and other laboratory testing

Method Name

Bead-Based Multiplex Immunoassay

Reporting Name

Th1 Cytokine Panel, P

Specimen Type

Plasma EDTA

Specimen Minimum Volume

0.3 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Plasma EDTA Frozen 21 days

Reject Due To

Gross hemolysis Reject
Gross lipemia Reject
Gross icterus Reject
Heat-treated specimen Reject

Clinical Information

Cytokines are important mediators of cell-to-cell communication within the innate and adaptive immune systems.  The expression of most cytokines is highly regulated, and generally occurs in response to foreign or self-antigenic stimulation. The functions of cytokines are extremely varied, with many cytokines also displaying pleiotropic effects, depending on their cellular target. As a group, cytokines and their receptors represent a highly complex and critical regulator of a normal immune response.

Reference Values

TNF:

<18 years: Not established

≥18 years: <10.0 pg/mL

 

IFN-gamma:

<18 years: Not established

≥18 years: <60.0 pg/mL

 

IL-2:

<18 years: Not established

≥18 years: <60.0 pg/mL

 

IL-2 receptor alpha soluble:

<18 years: Not established

≥18 years: ≤959 pg/mL

 

IL-12p70:

<18 years: Not established

≥18 years: <200 pg/mL

Interpretation

Elevated cytokine concentrations could be consistent with the presence of infection or other inflammatory process.

Cautions

Results from cytokine testing should not be used to establish or exclude a specific diagnosis.

 

Cytokine testing should only be used in conjunction with clinical information and other laboratory testing as part of a patient's overall assessment.

 

Normal concentrations of cytokines do not exclude the possibility of infection or other inflammatory condition.

 

Cytokine concentrations could be affected by immunomodulatory agents.

Clinical Reference

1. Cerrillo E, Moret I, Iborra M, et al. A nomogram combining fecal calprotectin levels and plasma cytokine profiles for individual prediction of postoperative Crohn's disease recurrence. Inflamm Bowel Dis. 2019;25(10):1681-1691. doi:10.1093/ibd/izz053

2. Li S, Hao X, Gong Y, et al. Effect of shenling baizhu powder on the serum TH1 cytokines of elderly patients with ulcerative colitis complicated by bloody purulent stool. Am J Transl Res. 2021;13(8):9701-9707

3. Abdelrahman AH, Salama, II, Salama SI, et al. Role of some serum biomarkers in the early detection of diabetic cardiomyopathy. Future Sci OA. 2021;7(5):FSO682. doi:10.2144/fsoa-2020-0184

4. Weng L, Chen Y, Liang T, et al. Biomarkers of interstitial lung disease associated with primary Sjogren's syndrome. Eur J Med Res. 2022;27(1):199. doi:10.1186/s40001-022-00828-3

5. Popova V, Geneva-Popova M, Kraev K, Batalov A. Assessment of TNF-alpha expression in unstable atherosclerotic plaques, serum IL-6 and TNF-alpha levels in patients with acute coronary syndrome and rheumatoid arthritis. Rheumatol Int. 2022;42(9):1589-1596. doi:10.1007/s00296-022-05113-4

6. Li L, Chen B, Zhao H, Wang G. Bone changes and curative effect of infliximab in patients with ankylosing spondylitis. J Musculoskelet Neuronal Interact. 2020;20(3):437-443

7. Zhao S, Liu Y, Zhou C, et al. Prediction model of delayed graft function based on clinical characteristics combined with serum IL-2 levels. BMC Nephrol. 2022;23(1):284. doi:10.1186/s12882-022-02908-2

8. Ye Q, Shao WX, Xu XJ, Yang YZ. The clinical application value of cytokines in treating infectious diseases. PLoS One. 2014;9(6):e98745. doi:10.1371/journal.pone.0098745

9. Wang Q, Wang C, Yang M, Li X, Cui J, Wang C. Diagnostic efficacy of serum cytokines and chemokines in patients with candidemia and bacteremia. Cytokine. 2020;130:155081. doi:10.1016/j.cyto.2020.155081

Method Description

Testing for plasma cytokine levels is accomplished using a laboratory-developed immunoassay.(Unpublished Mayo method)

Day(s) Performed

Thursday

Report Available

2 to 8 days

Specimen Retention Time

14 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83520 x5

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CYTH1 Th1 Cytokine Panel, P 82335-1

 

Result ID Test Result Name Result LOINC Value
622037 TNF 3074-2
622038 IFN-gamma 27415-9
622039 IL-2 33939-0
622040 IL-2 receptor alpha soluble 76039-7
622041 IL-12p70 41760-0

NY State Approved

Yes