Test Code LAB15186 KLEIHAUER TEST, BLOOD
Specimen Information
| Container | Specimen | Temperature | Collect Vol | Submit Vol | Min Vol | Stability | 
| Lav Top Tube | Whole Blood | Refrigerate | 4 mL | 4 mL | 1.5 mL | 7 days | 
Test Schedule / Analytical Time / Test Priority
Monday – Friday 7 am to 3:30 only / 1 day / Not available STAT
Method
Acid Elution of Adult Hemoglobin
CPT(s)
| Description | CPT Code | 
| Kleihauer Test, Blood | 85460 | 
Instrumentation
Manual Method
Reference Range
Negative
Section
Hematology
Performing Location
University of Vermont Medical Center
Is the UVMMC lab NY State Certified to perform this testing? Yes/No
Yes
Result Components
| Reporting Name | Epic Code | Atlas Code | Mayo Access ID | LOINC | 
| Kleihauer Test | 12301011923 | LRR11923 | FAH5944 | 32140-6 | 
| mL F/M Hemorrhage | 12301011924 | LRR11924 | FAH5945 | 48555-7 | 
| Dose of RhIg | 12301011925 | LRR11925 | FAH5946 | 1313-6 | 
Clinical Indications
| Definitely Indicated | Clinical Judgment | Not Indicated | 
| RhIG (if positive fetal screen) | Maternal Trauma; External cephalic version | 
 | 
| Fetal loss > 20wks | Neonatal Anemia | Non-traumatic abruption | 
| Fetal hydrops | Cord blood sample | Vaginal bleeding | 
| High clinical concern for massive fetal maternal hemorrhage with MCA dopplers | 
 | 
If the patient is Rh (-) a Fetal Screen Test should be initially ordered. A Kleihauer-Betke will automatically be reflexed and run on all positive fetal screens.
The Kleihauer-Betke (KB) test is designed to quantitate fetal red blood cells, but the results have been shown to be imprecise with a large coefficient of variation (CV). It is often utilized to quantitate large fetomaternal hemorrhages at the time of delivery in Rh (-) women for appropriate dosing of RhIG to prevent Rh alloimmunization. Although it can be used during pregnancy in both Rh (-) and Rh (+) women to detect fetomaternal hemorrhage, the evidence to support its utility are limited and results should be interpreted with caution.
 
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