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Test Code LAB239 BACTERIAL CULTURE, URINE

Method

Culture

CPT Code(s)

Description CPT Code
Bacterial Culture, Urine                                    87086

 

Specimen Information

Container Specimen Temperature Collect Vol Submit Vol Min Vol Stability
Sterile Container Clean Catch Urine Refrigerate 20 mL 10 mL 1 mL *
Gray Top w/boric acid Clean Catch Urine Ambient 20 mL 20 mL 3 mL 48 hours

*Deliver to lab immediately. If the specimen cannot be processed within one hour, refrigerate the specimen at 4oC. Bacterial counts will remain stable at 4oC for 24 hours. Culture will not be performed on urine samples that are refrigerated greater than 24 hours.

Reference Range

 

No growth or usual urogenital flora

  • Colony counts are done using a calibrated loop. Colony counts are reported based on 2 reference points: 10,000 CFU/mL and 100,000 CFU/mL.
  • Organisms are identified and susceptibility testing is done (when indicated), when more than 10,000 CFU/mL of 1 or 2 pathogens are present.
  • A sample with 3 or more potential pathogens is reported as:Mixed organisms, Interpretation difficult. Multiple gram positive organisms representing skin flora or vaginal contamination will be reported  as usual urogenital flora.
  • No growth reports are finaled between 18 and 48 hours depending upon specimen receipt.  Organism identification and susceptibility testing results are completed within 48-72 hours.

Instrumentation

Manual Method

Performing Location

University of Vermont Medical Center

Test Schedule / Analytical Time / Test Priority

Daily / Reported when positive. Negatives finaled 18-48 hours / Not available STAT

Section

Microbiology-1

Is the UVMMC lab NY State Certified to perform this testing?  Yes/No

Yes