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Test Code UHSD2 Hemosiderin, Random, Urine


Specimen Required


Container/Tube: Plastic urine container

Specimen Volume: 13 mL

Collection Instructions:

1. Collect a random urine specimen.

2. No preservative.


Useful For

Detecting hemosiderinuria, secondary to excess hemolysis, as in incompatible blood transfusions, severe acute hemolytic anemia, or hemochromatosis for external patients.

Method Name

Rous Method 

Reporting Name

Hemosiderin, Random, U

Specimen Type

Urine

Specimen Minimum Volume

12 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 7 days
  Frozen  7 days
  Ambient  2 hours

Reject Due To

  All specimens will be evaluated at Mayo Clinic Laboratories for test suitability.

Clinical Information

When the plasma hemoglobin level is 50 to 200 mg/dL after hemolysis, the capacity of haptoglobin to bind hemoglobin is exceeded, and hemoglobin readily passes through the glomeruli of the kidney. Part of the hemoglobin is absorbed by the proximal tubular cells where the hemoglobin iron is converted to hemosiderin. When these tubular cells are later shed into the urine, hemosiderinuria results. If the hemoglobin cannot be absorbed into the tubular cells, hemoglobinuria results.

 

Hemosiderin is found as yellow-brown granules that are free or in epithelial cells and occasionally in casts in an acidic or neutral urine.

Reference Values

Negative

Interpretation

A positive hemosiderin indicates excess red cell destruction.

Clinical Reference

1. Brunzel N. Chemical examination of urine. Fundamentals of Urine and Body Fluid Analysis. 4th ed. Saunders; 2018:98-99

2. Henry JB. Clinical Diagnosis and Management by Laboratory Methods. 18th ed. WB Saunders Company; 1991:412-413

3. Cappellini MD, Lo SF, Swinkles DW. Hemoglobin, iron, bilirubin. In: Rifai N, Horvath AR, Wittwer CT, eds. Tietz Textbook of Clinical Chemistry and Molecular Diagnostics. 6th ed. Elsevier; 2018:747

Method Description

The Prussian blue reaction is used to demonstrate hemosiderin as first described by Rous in 1918.(Brunzel N. Microscopic examination of urine sediment. Fundamentals of Urine and Body Fluid Analysis. 4th ed. Saunders; 2018:392)

Day(s) Performed

Monday through Sunday

Report Available

1 day

Specimen Retention Time

2 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

83070

LOINC Code Information

Test ID Test Order Name Order LOINC Value
UHSD2 Hemosiderin, Random, U 4644-1

 

Result ID Test Result Name Result LOINC Value
HSDU2 Hemosiderin, Random, U 4644-1

NY State Approved

Yes