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Test Code VB12 VITAMIN B12

Important Note

The results of this assay can be falsely elevated due to the consumption of Biotin. Patients should discontinue the use of vitamins or supplements that contain Biotin 12 hours before blood draw. See Resources for more information.

Performing Laboratory

Porter Hospital Laboratory

Specimen Requirements

Specimen Type: Blood
Container/Tube: Tiger top, serum gel

Specimen Volume: Full tube, submit 0.5 mL serum
Specimen Minimum Volume: 0.35mL
Collection Instructions:
1. Send specimen in original tube.
2. If there is a delay in transport of >1 hour, centrifuge tube.
3. Sample must be separated with 2 hours of collection.
4. Centrifuged samples are stable for:
• 3 days at 2 – 8°C

Specimen Transport Temperature


Days Performed


Test Classification and CPT Coding


Normal Reference Values

193-986 pg/mL

Critical Values


Specimen Requirements Additional Information

Serum – Spin within 2 hours after collection.